First Post! =D

i thought it would be a good idea to start this =D

15/06/07 - Went to the lab at 8. Darren was alrdy there (surprise surprise :P) and we got our PCR stuffs ready. TODAY'S AIM: to produce a perfect PCR. Our previous flops were either a positive negative (o.O), or poor sample bands (30 cycles nya), or no bands at all(heat extraction) and some were becoz we tested other primers (GA for type II, SP for type IV). For the records and our log book, both were negative.

So... 7 samples, one F+ and one somatic. and our ever optimistic negative. Darren did the PCR yesterday; my jobs were to get all the stuff and to kaypoh the HERV and veggies group. The veggies grp came for one hour nya, but they do it everyday =D so we're not the only fierce ones =D

OUR RESULTS (after a 60 min gel) : The somatic fella was amplifying like the F+ fella and the F+ fella decided to amplify like a somatic......F+ is supposed to be at 204 kbp and somatic is less... our results were showing something different. FURTHERMORE.... our negative decided to be optimistic today and therefore giving us a very nice band...After getting some SOS, it was concluded that we MAY have mixed up F+ with somatic during our first extraction. Therefore, we extracted our F+ again. and we gave the gel another 30 mins to run.For fun.

OUR RESULTS (after a for fun 90 min gel) : Somehow the F+ and somatic swopped lanes in extra time! What the beep =D so everything turned out ok in extra-time. except for our "negative". but we continued to extract the F+ anyway. come monday, it will be Darren's Pos VS AhDriel's Pos, and one somatic pos, and Water negative pos VS nothing negative pos. OBJECTIVE: to see whether Darrens Pos is really a F+ and not a somatic, and to see whether the water used in negative is contaminated =D

Gunning for Best Project? =D